Comparison of Different PCR Approaches for Typing of Francisella tularensis Strains

Genotyping of Francisella Tularensis Strains Isolated from Different Geographical Regions

Simple and Rapid Detection of Yersinia Pestis and Francisella Tularensis using Multiplex-PCR

Background: Yersinia pestis and Francisella tularensis cause plague and tularemia, which are known as diseases of the newborn and elderly, respectively. Immunological and culture-based detection methods of these bacteria are time-consuming, costly, complicated and require advanced equipment. We aimed to design and synthesize a gene structure as positive control for molecular detection of these ...

Genomic markers for differentiation of Francisella tularensis subsp. tularensis A.I and A.II strains.

Tularemia is caused by two subspecies of Francisella tularensis, F. tularensis subsp. tularensis (type A) and F. tularensis subsp. holarctica (type B). F. tularensis subsp. tularensis is further subdivided into two genetically distinct populations (A.I and A.II) that differ with respect to geographical location, anatomical source of recovered isolates, and disease outcome. Using two human clini...

Real-time PCR for Francisella tularensis Types A and B

Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 11, November 2006 1799 6.4 nmol/L and that showed an H243Y mutant type codon. The role of such a polymorphism appears unclear. We found no association between the 3-bp indel in G7 and in vitro dhART susceptibility because mutants were regularly distributed in highly susceptible isolates and in isolates having a diminished susceptibil...

Francisella tularensis molecular typing using differential insertion sequence amplification.

Tularemia is a potentially fatal disease that is caused by the highly infectious and zoonotic pathogen Francisella tularensis. Despite the monomorphic nature of sequenced F. tularensis genomes, there is a significant degree of plasticity in the organization of genetic elements. The observed variability in these genomes is due primarily to the transposition of direct repeats and insertion sequen...